SPRIselec试剂盒基于SPRI的磁珠专利技术，用于NGS核酸特定片段范围筛选!不仅保证了重复性高的实验结果，还可以自由选择需要筛选的片段范围。SPRIselectisaSPRI-basedchemistrythatspeedsandsimplifiesnucleicacidsizeselectionforfragmentlibrarypreparationforNextGenerationsequencing。
AlowervolumewilldecreasepipettingaccuracyofSPRIselect,thereforeincreasingselectionpointvariability！•DNAfragmentsmaybesizeselectedinarangenosmallerthan150bpandnolargerthan800bp！•TomaximizerecoveryforaLeftSideSizeSelection,themajorityofthesample’ssizedistributionshouldbelargerthantheselectionpoint!
Inthisprocess,sizeselectionisrequiredtoproduceauniformdistributionoffragmentsaroundanaeragesize！UsingSPRIselect,thesizedistributioncanbeadjustedtosuittheapplicationandplatformused！Theprocesscanbescaledforlowtohighthroughputworkflows。
货品编号：B23317类型：SPRIselect试剂盒规格：5ml商品效期：以产品标识实际效期为准贮藏条件：需18-25℃贮藏Materials•85%Ethanol,non-denatured(ethanolishygroscopic,preparefreshforoptimalresults)•Water(molecularbiologygrade)orastandardbuffersolutionsuchasTris(10mM,pH8)orTE(10mMTris,pH8,1mMEDTA)forDNAelutionSamplePrerequisites•Samplesshouldbefragmenteddouble-strandedDNA！
核酸筛选试剂盒B23317
•TomaximizerecoveryforaRightSideSizeSelection,themajorityofthesample’ssizedistributionshouldbesmallerthantheselectionpoint!•TomaximizerecoveryforaDoubleSizeSelection,thesizedistributionshouldbecenteredbetweentheselectionpoints。
•SamplesshouldbedissolvedinmolecularbiologygradewaterorstandardbuffersolutionsuchasTrisorTE！SeeEffectsofCommonLaboratoryReagentsonSizeSelectionatendofuserguideformoreinformation!•Samplevolumeshouldbe≥50μL!
Theguidanceprovidedbelowcanbeusedtooptimizethedesiredsizeselectionrange！UsedmanuallyorautomatedonaliquidhandlingsystemsuchastheBiomekLaboratoryAutomationWorkstation,SPRIselectwillproviderapidandconsistentsizeselectionsuitableformostapplications!
