•TomaximizerecoveryforaRightSideSizeSelection,themajorityofthesample’ssizedistributionshouldbesmallerthantheselectionpoint。•TomaximizerecoveryforaDoubleSizeSelection,thesizedistributionshouldbecenteredbetweentheselectionpoints!
Inthisprocess,sizeselectionisrequiredtoproduceauniformdistributionoffragmentsaroundanaeragesize。UsingSPRIselect,thesizedistributioncanbeadjustedtosuittheapplicationandplatformused.Theprocesscanbescaledforlowtohighthroughputworkflows。
AlowervolumewilldecreasepipettingaccuracyofSPRIselect,thereforeincreasingselectionpointvariability!•DNAfragmentsmaybesizeselectedinarangenosmallerthan150bpandnolargerthan800bp!•TomaximizerecoveryforaLeftSideSizeSelection,themajorityofthesample’ssizedistributionshouldbelargerthantheselectionpoint。
Theguidanceprovidedbelowcanbeusedtooptimizethedesiredsizeselectionrange！UsedmanuallyorautomatedonaliquidhandlingsystemsuchastheBiomekLaboratoryAutomationWorkstation,SPRIselectwillproviderapidandconsistentsizeselectionsuitableformostapplications!
磁珠法筛选核酸B23317
•SamplesshouldbedissolvedinmolecularbiologygradewaterorstandardbuffersolutionsuchasTrisorTE。SeeEffectsofCommonLaboratoryReagentsonSizeSelectionatendofuserguideformoreinformation!•Samplevolumeshouldbe≥50μL.
货品编号：B23317类型：SPRIselect试剂盒规格：5ml商品效期：以产品标识实际效期为准贮藏条件：需18-25℃贮藏Materials•85%Ethanol,non-denatured(ethanolishygroscopic,preparefreshforoptimalresults)•Water(molecularbiologygrade)orastandardbuffersolutionsuchasTris(10mM,pH8)orTE(10mMTris,pH8,1mMEDTA)forDNAelutionSamplePrerequisites•Samplesshouldbefragmenteddouble-strandedDNA。
